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| Registros recuperados: 225 | |
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| Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),... |
| Tipo: Text |
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf |
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| Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas. |
| Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the... |
| Tipo: Text |
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck. |
| Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the... |
| Tipo: Text |
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf |
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| Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy. |
| The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are... |
| Tipo: Text |
Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf |
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| Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy. |
| The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement... |
| Tipo: Text |
Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf |
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| Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene. |
| Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf |
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| Lallias, Delphine. |
| Cette étude vise à approfondir la connaissance de la biologie de la reproduction de l'huître plate Ostrea edulis afin de mieux comprendre comment les caractéristiques de la biologie de reproduction de l'espèce peuvent rendre compte des phénomènes de déficits en hétérozygote et de relation positive entre hétérozygotie et croissance. En particulier, elle vise à analyser le succès reproducteur en milieu naturel et dans les populations maintenues en écloserie. Cette étude repose sur l'utilisation de marqueurs microsatellites qui, du fait de leur fort polymorphisme, représentent des outils puissants d'assignation de parenté. Une première partie de l'étude a consisté à analyser les pontes issues de femelles incubantes prélevées dans le milieu naturel. Le nombre... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Microsatellites; Biologie de la reproduction; Variance du succès reproducteur. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00370/48167/48281.pdf |
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| Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre. |
| Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between... |
| Tipo: Text |
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf |
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| Boudry, Pierre; Launey, Sophie; Huvet, Arnaud. |
| The two common oyster species cultivated in Europe are the flat oyster Ostrea edulis and the cupped oyster Crassostrea gigas. These species have two really different reproduction strategies (oviparity versus larviparity) and histories (natural distribution versus introductions linked to oyster culture). We used microsatellite markers to compare the observed variabilities Europe wide. O. edulis presents a lower rate of alleles per locus than C. gigas. The observed structuration levels (global Fst and by pairs values) are similar for both species, but the spatial organisation of the diversity is different. In O. edulis, appears a differentiation based on the distance (concordance of the results with those obtained with the allozymes) whereas in C. gigas,... |
| Tipo: Text |
Palavras-chave: Différenciation génétique; Variabilité; Génétique; Crassostrea gigas; Ostrea edulis; Huîtres. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-3455.pdf |
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| Denoix, Michael. |
| Depuis la fin des années soixante, la conchyliculture française doit faire face à des mortalités importantes d'huître plate, Ostrea edulis, causées entre autres, par le protozoaire, Bonamia ostreae, responsable de la bonamiose. Peu de moyens sont à l'heure actuelle à la disposition des professionnels pour lutter contre cette maladie. L'obtention d'animaux résistants à cette infection est une voie retenue et explorée par l'Ifremer pour combattre la bonamiose et tenter d'apporter des solutions aux conchyliculteurs. Dans cette optique, l'Ifremer, a entrepris un programme de sélection pour obtenir des animaux résistants en exerçant une pression de sélection par inoculation de parasites purifiés dans la cavité péricardique d'animaux anesthésiés. Les premières... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Hemocyte; Parasite. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00033/14443/11742.pdf |
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| Suquet, Marc; Pouvreau, Stephane; Queau, Isabelle; Boulais, Myrina; Le Grand, Jacqueline; Ratiskol, Dominique; Cosson, Jacky. |
| n brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe. Flat oysters were collected by divers in the Rade de Brest (Finistère, France), during its natural reproduction period. Gonadal pH is acidic (6.31 ± 0.10). Spermatozoa are clustered in spermatozeugmata, an acellular structure in which the sperm heads are embedded. After their transfer in seawater, spermatozeugmata have a mean diameter of 64 ± 3 μm and they release free spermatozoa for a mean duration of... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Brooding species; Sperm; Spermatozeugmata; Endangered species. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56539/58245.pdf |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf |
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| Registros recuperados: 225 | |
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